Test Order - Bordetella Pertussis Identification or Confirmation

Pre-Approval Required

No

Supplemental Information or Form

1. Pure, viable subculture
2. Completed submittal form with 2 patient identifiers (e.g. name and date of birth), collection date and source, a brief but complete patient history, cultural history and submitting laboratory’s findings
3. Documentation that indicates the role of this isolate in clinical disease
4. Form LAB​ 446 (PDF)
   

Performed on Specimens From

None

Acceptable Sample/Specimen Type for Testing

Suspected Bordetella pertussis isolate

Minimum Volume Required

None

Storage & Preservation of Sample/Specimen Prior to Shipping

None

Transport

Tubed agar medium with a secure screw cap, labeled with patient’s name.  Alternatively, blocks of Regan-Lowe or Bordet-Gengou agar may be aseptically be cut from plates and then inserted into tubed agar medium of another type with a secure screw cap.

Sample/Specimen Labeling

None

Shipping Instructions which Include Specimen Handling Requirements

1. Grow isolate under appropriate conditions prior to shipping.
2. Ship at ambient temperature using appropriate DOT/IATA approved shipping procedures.

Test Methodology

As needed - Biochemical testing, species specific slide agglutination, Gene Sequencing by PCR, and/ or MALDI-TOF mass spectrometry

Turnaround Time

Two weeks

Interferences & Limitations

Mixed or nonviable culture

Additional Information

Please contact the MDL Vaccine Preventable Pathogens & Molecular Bacteriology Unit (VPP & MBU) for special consideration if any of the above criteria cannot be met.

MDL Points of Contact