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VIRAL AND RICKETTSIAL DISEASE LABORATORY

Test Order
Dengue Virus Detection - PCR 

Information Category Information Detail
Pre-Approval None
Supplemental Information Requested
 Requested: Travel history, illness onset date, collection date
Submittal Form Specimens must be accompanied with a hard copy of the completed VRDL General Purpose Specimen Submittal Formā€‹ā€‹ (PDF) or a form generated in the VRDL Lab Web Portal.
Methodology (Commercial Test Name or Laboratory Developed Test (LDT)) CDC EUA Trioplex Real-time RT-PCR Assay (Diagnostic)
Reflex Testing None
Acceptable Specimen Type(s) and Collection Method

Human specimens:

  • Serum:
    • ā€‹ā€‹ā€‹ā€‹Plain Red-Top Tubes: Serum collected in plain, red-top tubes must be separated and transferred to sterile, screw-cap tubes for storage and transport.
    • Serum Separator Tubes (SST): SST may be transported after centrifugation.
  • Whole Blood: EDTA (lavender top) Tubes
  • CSF: Collect CSF in sterile, screw-cap tubes.
Minimum Volume Requested
  • Serum: 1 mLā€‹
  • Whole Blood in EDTA Tubes: 5 mL
  • CSF: 1 mL
Transport Medium (if using) Not Applicable
Specimen Labeling Each specimen tube must be labeled with at least two unique patient identifiers, e.g., patient full name and date of birth.
Storage & Preservation of Specimen / Shipping Conditions
  • Separated Serum: Refrigerate or freeze separated serum promptly after collection.
  • Serum Separator Tubes: Refrigerate, do not freeze.
  • Whole Blood: Refrigerate, do not freeze.
  • CSF: Refrigerate or freeze CSF promptly after collection.ā€‹

Ship refrigerated specimens to VRDL on cold packs. Ship frozen specimens to VRDL on dry ice.

Shipping Instructions

 

Work with your local public health department to ensure samples are packaged according to instructions for Biological Substance ā€“ Category B (UN 3373) shipment.

Ship specimens and a hard copy of the completed submittal forms to:    

CDPH VRDL

ATTN: Specimen Receiving

850 Marina Bay Parkway

Richmond, CA 94804

Phone: 510-307-8585
Turnaround Time 10 Business Days
Limitations Negative result does not preclude the possibility of infection.
Additional Information Specimens should be collected as close to onset as possible.
VRDL Points of Contact Medical and Epidemiology Liaisons: (510) 307-8585 or VRDL.submittal@cdph.ca.gov

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