Pre-Approval
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None |
| Supplemental Information Requested |
Requested: Travel history, illness onset date |
Submittal Formāā
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Specimens must be accompanied with a hard copy of the completed
VRDL General Purpose Specimen Submittal Formāā (PDF) or a form generated in the
VRDL Lab Web Portal. |
| Methodology (Commercial Test Name or Laboratory Developed Test (LDT)) |
EIA: IgM (Commercial Test)(Diagnostic)
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| Reflex Testing |
Flavivirus Plaque Reduction Neutralization Test |
| Acceptable Specimen Type(s) and Collection Method |
Human specimens:
- āāSerum:
- Plain Red-Top Tubes: Serum collected in plain, red-top tubes must be separated and transferred to sterile, screw-cap tubes for storage and transport.
- Serum Separator Tubes (SST): SST may be transported after centrifugation.ā
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Minimum Volume Requestedā
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| Transport Medium (if using) |
Not Applicable |
| Specimen Labeling |
Each specimen tube must be labeled with at least two unique patient identifiers, e.g., patient full name and date of birth.
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| Storage & Preservation of Specimen / Shipping Conditions |
- āSeparated Serum: Refrigerate or freeze separated serum promptly after collection.
- Serum Separator Tubes: Refrigerate, do not freeze.
Ship refrigerated specimens to VRDL on cold packs. Ship frozen specimens to VRDL on dry ice.
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| Shipping Instructions |
Work with your local public health department to ensure samples are packaged according to instructions for Biological Substance ā Category B (UN 3373) shipment.
Ship specimens and a hard copy of the completed submittal forms to:
āCDPH VRDL
ATTN:
Specimen Receiving
850 Marina Bay Parkway
Richmond, CA 94804
Phone: 510-307-8585
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| Turnaround Time |
10 Business Days |
| Limitations |
Serologic assays do not distinguish between dengue serotypes.
Positive result does not confirm infection, supplemental testing is required.āā Negative result does not preclude the possibility of infection.
Flavivirus antibodies are often cross-reactive.
Cross-reactivity with Malaria IgM has not been evaluated with the DENV Detect IgM Capture ELISA.
Results from immunosuppressed patients must be interpreted with caution.
High cholesterol levels (>300 mg/dL) appear to give variable results and may affect the DENRA OD values.
High triglyceride levels (>3000 mg/dL) appeared to exhibit a slight effect of raising the ISRs of low positive sera.
Hemoglobin (>1600 mg/dL) appears to affect some serum samples by lowering the ISRs.
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| Additional Information |
A convalescent specimen is recommended to help determine recency of infection. When submitted separately, be sure to identify the previously submitted specimen by the VRDL accession number so that the acute and convalescent specimens may be linked, tested to endpoint and interpreted as a pair. |
| VRDL Points of Contact |
Medical and Epidemiology Liaisons: (510) 307-8585 or
VRDL.submittal@cdph.ca.govāāā
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