Pre-Approval
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Not Applicable
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āSupplemental Information Requested
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Requested: Travel history, rash onset date, symptoms, measles vaccination history, contacts to known case(s)
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āSubmittal Form
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Specimens must be accompanied with a hard copy of the completed VRDL General Purpose Specimen Submittal Formāā (PDF) or a form generated in the VRDL Lab Web Portal.ā
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Methodology
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Sequence-based genotyping (LDT) (Surveillance use only)
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Reflex Testing
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May be reflexed to whole genome sequencing
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āAcceptable Specimen Type(s) and Collection Method
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Human specimens:
- Respiratory specimens: Throat (preferred) and/or NP swabs in sterile collection tube with liquid viral transport media
- Urine: Processed urine preferred
- Measles-positive nucleic acid extractsā
Respiratory swabs must be a Dacron or synthetic tip with aluminum or plastic shaft.
To process urine, centrifuge urine sample (10-50 mL) at 500-600 x g for 5-10 min at 2-8Ā°C. Discard supernatant and resuspend cell sediment in 2-3 mL of viral transport media.
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āMinimum Volume Requested
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- Respiratory swab: 2ā3 mL of Universal or Viral Transport Medium
- Urine, unprocessed: 10-50 mL
- Urine, processed: Pellet resuspended in 2ā3 mL of Universal or Viral Transport Medium
- Nucleic acid extracts: 25-50 ĀµL
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āTransport Medium (if using)
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For respiratory swabs only: 2-3 mL of Universal or Viral Transport Medium
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āSpecimen Labeling
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āEach specimen tube must be labeled with at least two unique patient identifiers, e.g., patient full name and date of birth.
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āStorage & Preservation of Specimen / Shipping Conditions
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- Respiratory swabs and urine pellets: freeze or refrigerate specimens promptly after collection.
- For unprocessed urine, refrigerate specimens promptly after collection. Do not freeze unprocessed urine.
Ship refrigerated specimens to VRDL on cold packs. Ship frozen specimens to VRDL on dry ice.
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āShipping Instructions
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Work with your local public health department to ensure samples are packaged according to instructions for Biological Substance ā Category B (UN 3373) shipment.
Ship specimens and a hard copy of the completed submittal forms to:
āCDPH VRDL
ATTN:
Specimen Receiving
850 Marina Bay Parkway
Richmond, CA 94804
Phone: 510-307-8585
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Turnaround Time
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10 Business Days
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Limitations
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āNegative results do not preclude the possibility of infection.
This test may not be used for diagnosis.
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āAdditional Information
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Specimens should be collected as close to symptom onset as possible.
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āVRDL Points of Contact
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Medical and Epidemiology Liaisons: (510) 307-8585 or VRDL.submittal@cdph.ca.govāāā
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