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VIRAL AND RICKETTSIAL DISEASE LABORATORY

Test Order
Monkeypox Virus (Mpox) Detection - PCR

ā€‹Information Category
ā€‹Information Detail
Pre-Approval

Not Applicable

ā€‹Supplemental Information Requested
Not Applicable
ā€‹Submittal Forms

Specimens must be accompanied with a hard copy of the completed VRDL General Purpose Specimen Submittal Formā€‹ (PDF)ā€‹ā€‹ or a form generated in the VRDL Lab Web Portal.ā€‹ā€‹

(Mpox group submittal form available on request from VRDL.Submittal@cdph.ca.gov)

Methodology 

Real-time PCR (LDT) (Diagnostic)ā€‹

Reflex Testing
  • May be reflexed to Non-variola Orthopox Virus
  • May be reflexed to Varicella Zoster and/or Herpes Simplex Virus
  • May be referred to CDC for additional testing
ā€‹Acceptable Specimen Type(s) and Collection Method
Human Specimens:
  • Lesion swabs, in Viral Transport Medium (preferred) or dry
  • Dry lesion scabs or crusts
ā€‹Minimum Volume Requested
Submitters should sample at least  two (2) Mpox lesions per patient (maximum 4 lesions). Each lesion should be sampled with 2 separate swabs to accommodate confirmatory testing, if needed. 
ā€‹Transport Medium (if using)
For lesion swabs only: 1ā€“3 mL of Viral Transport Medium
ā€‹Specimen Labeling
ā€‹Each specimen tube must be labeled with at least two unique patient identifiers; e.g., patient full name and date of birth.
ā€‹Storage & Preservation of Specimen / Shipping Conditions
  • Lesion swabs: freeze or refrigerate specimens promptly after collection. Freezing is strongly recommended.

Ship refrigerated specimens to VRDL on cold packs. Ship frozen specimens to VRDL on dry ice.

ā€‹Shipping Instructionsā€‹
Work with your local public health department to ensure samples are packaged according to instructions for Biological Substance ā€“ Category B (UN 3373) shipment.  

Ship specimens and a hard copy of the completed submittal forms to:
     CDPH VRDL

     ATTN: Specimen Receiving

     850 Marina Bay Parkway
     Richmond, CA 94804
     Phone: 510-307-8585
Turnaround Time
5 business days
Interferences & ā€‹Limitations
ā€‹Negative results do not preclude the possibility of infection.
ā€‹Additional Information
ā€‹This assay can distinguish between Mpox Clades I and II viruses. 

CDPH Mpox Disease Information  
ā€‹
ā€‹VRDL Points of Contact
Medical and Epidemiology Liaisons: (510) 307-8585 or VRDL.Submittal@cdph.ca.govā€‹ 
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